| 產品名稱 |
CL4-1 [T-ALK] |
| 商品貨號 |
B205266 |
| Designations |
CL4-1 [T-ALK] |
| GenBank Number |
D50516
U20165
|
| Species |
Homo sapiens, human |
| Depositors |
HL Moses, M Kawabata |
| Applications |
contains sequence activin A receptor type II-like kinase |
| Vector |
Construct size (kb): 6.300000190734863 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: XhoI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ', <-
primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, <-
promoter: lac, <-
primer site: M13 reverse, <-
replicon: pMB1
marker(s): ampR, <-
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: ACVRLK*
Gene name: activin A receptor type II-like kinase
Contains complete coding sequence?: Y
Tissue: placenta, male caucasian fetus
Type of DNA: cDNA
Insert 5' end: XhoI
Insert 3' end: EcoRI
Insert size (kb): 3.3
Cross references: DNA Seq. Acc.: D50516
Nucleotides 1-3167 of the insert correspond to
nucleotides 373-3539 of D50516 with 100% identity.
DNA Seq. Acc.: U20165
Nucleotides 1-3167 of the insert correspond to
nucleotides 1-3167 of U20165. Insert lengths(kb): 3.299999952316284 Tissue: placenta, male caucasian fetus Gene product: activin A receptor type II-like kinase [ACVRLK*] Target Gene: activin A receptor type II-like kinase |
| Insert Size (kb) |
3.300 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI/XhoI--3.3, 3.0; PstI--3.1, 2.4, 0.8; BgII--6.3; XbaI--6.3; PvuII--2.6, 2.2, 1.5. Insert contains the following restriction sites (approximate kb from the 5' end): BglII--2.26; HincII--2.48; NcoI--0.08, 3.12; PstI--0.05, 0.84; PvuII--2.00; StuI--2.84; XbaI--0.63. Insert contains a poly-T cloning artifact between the XhoI site and the 5' end of the gene. |
| References |
Kawabata M, et al. Cloning of a novel type II serine/threonine kinase receptor through interaction with the type I transforming growth factor-beta receptor. J. Biol. Chem. 270: 5625-5630, 1995. PubMed: 7890683
|
