Restriction digests of the clone give the following sizes (kb): BamHI--4.4; EcoRI--4.4; SacI--4.4. The GAL10 RNA start site is disrupted, thus providing only one direction of transcription. Unless a start site for transcription is provided, little or no message should be made from inserts cloned into the GAL10 side of the promoter cassette. Do not use this side of the promoter unless you can insure that mRNA will be properly initiated by providing an RNA start site. The polylinker sites listed may not be unique in this vector. Plasmid containing a SacI-GST-GAL1/GAL10-XhoI cassette useful for making other constructions. |
