| 產品名稱 |
pGEM2Vk167 [pSVk167] |
| 商品貨號 |
B205361 |
| Designations |
pGEM2Vk167 [pSVk167] |
| Species |
Mus musculus, mouse |
| Depositors |
U Storb |
| Applications |
The T7 promoter is used to make anti-sense RNA. |
| Vector |
Construct size (kb): 3.200000047683716 |
| Insert |
DNA: cDNA Insert lengths(kb): 0.3100000023841858 Tissue: myeloma MOPC-167 cell line Gene product: immunoglobulin kappa chain V24 [IgkV24] |
| Insert Size (kb) |
0.310 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
The EcoRI + BamHI double digest generates a 324 bp fragment by cutting within the polylinkers. Restriction digests of the clone give the following sizes (kb): EcoRI/BamHI--2.9, 0.3; BglI--3.2; BglII--uncut; HindIII--3.2. The antibodies encoded by myeloma MOPC-167 are specific to phosphorylcholine. The insert was cloned from p167kappa RI, and spans the leader--Vk167 region. The T7 promoter is used to make anti-sense RNA. |
| Classification |
Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
| References |
Storb U, et al. Trangenic mice with mu and kappa genes encoding antiphosphorylcholine antibodies. J. Exp. Med. 164: 627-641, 1986. PubMed: 2425036
Deanna Haasch, personal communication
|
