| 產品名稱 |
pcDNAI-ALDO4 |
| 商品貨號 |
B205603 |
| Designations |
pcDNAI-ALDO4 |
| GenBank Number |
S64136
|
| Species |
Rattus norvegicus, rat |
| Depositors |
CE Gomez-Sanchez, M Zhou |
| Applications |
in another host, produces protein cytochrome P450, subfamily XIB, polypeptide 2 (steroid-11-beta-hydroxylase, aldosterone synthase) Cytochrome P450, subfamily XIB, polypeptide 2 (aldosterone synthase), aldosterone synthase, steroid 11-beta/18-hydroxylase, steroid-11-beta-hydroxylase, 11-beta-hydroxylase |
| Vector |
Construct size (kb): 10.0 |
| Insert |
DNA: cDNA Insert lengths(kb): 3.0 Gene product: cytochrome P450, subfamily XIB, polypeptide 2 (steroid-11-beta-hydroxylase, aldosterone synthase)( Cytochrome P450, subfamily XIB, polypeptide 2 (aldosterone synthase), aldosterone synthase, steroid 11-beta/18-hydroxylase, steroid-11-beta-hydroxylase, 11-beta-hydroxylase) [CYP11B2] Target Gene: cytochrome P450, subfamily XIB, polypeptide 2 (steroid-11-beta-hydroxylase, aldosterone synthase) |
| Insert Size (kb) |
3.0 |
| Media |
ATCC® Medium 1235: LB Medium (ATCC medium 1065) with 5 mcg/ml tetracycline and 10 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): BstXI/NotI--4.4, 2.0, 1.35; BamHI--5.6, 2.3; HindIII--6.6, 1.2; PvuII--2.5, 1.7, 1.6, 0.7, 0.5, 0.3, 0.25; XhoI--5.6, 2.3. The insert contains the following restriction sites (nt from the 5' end): XhoI--848; HindIII--1035; HincII--1257; PvuII--1348, 1957, 2404, 2624; BamHI--2131; BstXI--1233. Contains the complete coding sequence (nt 292-1812). |
| References |
Zhou M, Gomez-Sanchez CE. Cloning and expression of a rat cytochrome P-450 11 beta-hydroxylase/aldosterone synthase (CYP11B2) cDNA variant. Biochem. Biophys. Res. Commun. 194: 112-117, 1993. PubMed: 8333830
Celso E Gomez-Sanchez, personal communication
|
