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The Keratinocyte Growth Kit contains components that when added to Dermal Cell Basal Medium (ATCC PCS-200-030) create a complete serum-free culture environment for keratinocytes. The complete keratinocyte growth medium is formulated to inhibit fibroblast growth, and the low calcium concentration (0.06 mM) slows differentiation. No feeder layers, extracellular matrix proteins or other substrates are required. The final concentration for each component in complete keratinocyte growth medium is as follows:
- Extract P: 0.4%
- rh TGF-alpha: 0.5 ng/mL
- L-Glutamine: 6 mM
- Hydrocortisone: 100 ng/mL
- Insulin: 5 µg/mL
- Epinephrine: 1.0 µM
- Apo-transferrin: 5 µg/mL
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