ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--3.0, 0.8, 0.35; BglI--2.3, 1.8; EcoRI--3.0, 1.1; PstI--4.1; PvuII--2.8, 1.05, 0.3.
Corresponds to the insert of lambdaBC1, which begins approximately at nt 1000 of the sequence record. Encodes the mature protein, but is missing part of the coding sequence for the amino terminus of the precursor.
Detects mRNA of approximately 2.5 kb in A172 glioblastoma and HT1080 fibrosarcoma cell lines.
References
Derynck R, et al. Human transforming growth factor-beta complementary DNA sequence and expression in normal and transformed cells. Nature 316: 701-705, 1985. PubMed: 3861940
