| Comments |
Eighty percent of plaques were detected by 32P-labeled total genomic DNA. Two negative plaques contained E. coli stuffer fragments from the arm preparation; stuffers are considered to be the most likely source of 'negative' plaques. Depth of sequence representation appears to be excellent. Contains the following 3 sequences from different parts of the X chromosome: p19-2, p58-1 (DXS14, ATCC 57128, 57129), and L1.28 (DXS7, ATCC 57302, 57303). When the library was screened with a HPRT cDNA probe, of 30600 plaques tested, 7 were positive after 2 cycles, and each showed a restriction map characteristic of HPRT. |
| References |
Djabali M, et al. The gene encoding L1, a neural adhesion molecule of the immunoglobulin family, is located on the X chromosome in mouse and man. Genomics 7: 587-593, 1990. PubMed: 2387585
Dietz-Band JN, et al. Isolation, characterization, and physical localization of 33 human X-chromosome RFLP markers. Cytogenet. Cell Genet. 54: 137-141, 1990. PubMed: 1979940
Lubahn DB, et al. Cloning of human andogen receptor complementary DNA and localization to the X chromosome. Science 240: 327-333, 1988. PubMed: 3353727
Larry L Deaven, personal communication
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