| 產品名稱 |
pSP65:ClVd#5 |
| 商品貨號 |
B205850 |
| Designations |
pSP65:ClVd#5 |
| Species |
Coleus viroid (ClVd) |
| Depositors |
RW Hammond, USDA - ARS |
| Vector |
Name of construct: pSP65:ClVd#5
Excise insert: EcoRI+HindIII
Size of construct (kb): 3.2
Markers: ampR
Name of vector: pSP65
Intact vector size: 3.010
Type of vector: plasmid
Vector end: EcoRI
Vector end: HindIII
Cloning sites: HindIII PstI SalI AccI HincII XbaI BamHI AvaI SmaI SacI
EcoRI
Polylinker sites: HindIII PstI SalI AccI HincII XbaI BamHI AvaI SmaI SacI
EcoRI
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
marker(s): ampR
promoter: SP6
replicon: pMB1
terminator: none |
| Insert |
Genome: Coleus viroid (ClVd)
Gene name: genome, partial
Contains complete coding sequence?: U
Type of DNA: cDNA
Insert end:
Insert end:
Insert size (kb): 0.2 |
| Insert Size (kb) |
0.2 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--3.1, 0.24; BglI--3.3; HindIII--3.3; PvuII--3.3. A PCR amplification product. |
| Classification |
Coleviroid |
| References |
Rosemarie W Hammond, personal communication
|
