Restriction digests of the clone give the following sizes (kb): BglI--2.0, 1.7; HindIII--3.7; XbaI--3.7. One of 9 shuttle vectors (ATCC 87585 - 87593) allowing lacZ detection of cloned inserts and containing the pUC19 MCS with all 10 cloning sites unique. Vectors differ in mode of replication (YE, YC or YI-type) and yeast marker (LEU2, URA3 or TRP1). The HindIII, PstI and the XbaI sites from the TRP1 gene of the S. cerevisiae were removed by using oligo-directed mutagenesis. |
