| 產品名稱 |
p17.4 |
| 商品貨號 |
B205906 |
| Designations |
p17.4 |
| GenBank Number |
X14951
|
| Species |
Mus musculus, mouse |
| Depositors |
RW Wilson |
| Vector |
Construct size (kb): 5.820000171661377 |
| Insert |
DNA: cDNA Insert lengths(kb): 2.855999946594238 Tissue: thymus Gene product: integrin, beta 2 (antigen CD18 (p95), lymphocyte function-associated antigen 1; macrophage antigen 1 (mac-1) beta subunit) [Cd18] |
| Insert Size (kb) |
2.856 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
The following are some of the enzymes which cut the insert only once: BamHI--2499; BglI--578; BglII--1668; BstEII--1310; DraI--2691; EcoRV--312; HindIII--2813, StuI--670. Restriction digests of the clone give the following sizes (kb): HindIII--2.9 (doublet); EcoRV--5.6, 0.3; BglII--6.0; EcoRI--2.9 (doublet). The open reading frame of 2313 nucleotides begins with ATG at nt 82 and ends with TAG at nt 2394. The insert is 2856 bp, and includes the TTATTTAT (nt 2679-2687) sequence found in inflammatory protein mRNAs. |
| Classification |
Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
| References |
Wilson RW, et al. Nucleotide sequence of the cDNA from the mouse leukocyte adhesion protein CD18. Nucleic Acids Res. 17: 5397, 1989. PubMed: 2569711
MacDonald GP, et al. Assignment of four human chromosome 21 genes to mouse chromosome 10: implications for mouse models of Down syndrome. Am. J. Hum. Genet. 43: A151, 1988.
Raymond W Wilson, personal communication
|
