| Vector |
Construct size (kb): 16.0 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBlueBac
Intact vector size: 14.000
Type of vector: phagemid
Vector end: NheI
Vector end: NheI
Cloning sites: NheI
Polylinker sites:
Host range: insect cells; Escherichia coli
Features (with orientation and position when available):
insert detection: lac
marker(s): ampR
promoter: polyhedrin
replicon: pMB1, f1, SV40
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: PRKCA
Other gene symbols: PKCA
Genomic copy number: unique
Gene name: protein kinase C, alpha
Contains complete coding sequence?: Y
Chromosome: 17; Localization: 17 q22-q24
Tissue: liver, HepG2 cell line
Type of DNA: cDNA
Insert end: SpeI
Insert end: SpeI
Insert size (kb): 2.0
Cross references: DNA Seq. Acc.: X52479 Insert lengths(kb): 2.0 Tissue: liver, HepG2 cell line Gene product: protein kinase C, alpha [PKCA] Target Gene: protein kinase C, alpha |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--10.0, 3.5, 1.3, 1.1, 0.25; BglI--5.8, 5.4, 2.2, 1.6, 1.2; NcoI--16.0; PstI--15.0, 0.65; PvuII--3.3, 2.5 (doublet), 2.3, 1.6, 1.4, 1.2, 0.75, 0.35. The insert contains the following restriction sites (approximate kb from the 5' end): BamHI--0.56, 0.77; BglI--0.94; NcoI--1.15; PstI--0.15; PvuII--1.21, 1.93; SmaI--0.02. Contains the complete coding sequence only (nt 28-2046 of GenBank X52479). The NheI/SpeI sites were destroyed during cloning. |
| References |
Finkenzeller G, et al. Sequence of human protein kinase C alpha. Nucleic Acids Res. 18: 2183, 1990. PubMed: 2336401
David J Burns, personal communication
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