| 產(chǎn)品名稱 |
pS3-1 |
| 商品貨號(hào) |
B206235 |
| Designations |
pS3-1 |
| GenBank Number |
M14168
|
| Species |
Homo sapiens, human |
| Depositors |
H Harris, PS Henthorn |
| Vector |
Construct size (kb): 6.199999809265137 |
| Insert |
DNA: cDNA Insert lengths(kb): 2.5 Tissue: osteosarcoma Saos-2 cell line Gene product: alkaline phosphatase, liver/bone/kidney [ALPL] Alleles: B1, B2, A1, C1, C2, D2, D1, A2 |
| Insert Size (kb) |
2.5 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--3.6, 2.6; BamHI--6.2; PstI--3.5, 2.5, 0.2. Restriction digests of the clone give the following sizes (kb): EcoRI--3.6, 2.55; BamHI--6.2; HindIII--6.2; PstI--3.5, 2.45; ClaI--6.2. Linkage has been established between ALPL and infantile hypophosphatasia (HOPS) in Manitoba Mennonite families (theta = 0, LOD score = 13.25). |
| References |
Weiss MJ, et al. Isolation and characterization of a cDNA encoding a human liver/bone/kidney-type alkaline phosphatase. Proc. Natl. Acad. Sci. USA 83: 7182-7186, 1986. PubMed: 3532105
Greenberg CR, et al. Infantile hypophosphatasia: localization within chromosome region 1p36.1-34 and prenatal diagnosis using linked DNA markers. Am. J. Hum. Genet. 46: 286-292, 1990. PubMed: 1689104
|
