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Distribution host: Escherichia coli JM109

Size (kb): 6.5000000000000000

DESCRIPTION OF VECTOR:
Intact vector size: 6.500
Type of vector: plasmid
Cloning sites: PstI HincII PvuI NcoI ScaI SspI BalI
Polylinker sites:
Construction: pDM2, pBGS8
Host range: Haemophilus influenzae; Escherichia coli
Features (with orientation and position when available):
marker(s): ampR, cmLR
replicon: pMB1, pRSF0885

Vector: pVT65 (plasmid)

Construction: pDM2, pBGS8

Marker(s):ampR,cmlR

Construct size (kb): 6.5

Features: marker(s): ampR, cmlR
replicon: pMB1, pRSF0885

shuttle vector

Restriction digests of the clone give the following sizes (kb): PstI--6.6; PvuI--6.6; SspI--3.7, 2.9; PvuII--6.2, 0.46; BamHI--4.0, 2.4.

Cloning into the HincII, PstI, PvuI, ScaI, or SspI sites inactivates ampR.

Cloning into the BalI or NcoI sites inactivates cmlR.

Precautions have not been taken to maintain the F' in the JM109 host. The F' is not required to use this plasmid.

Shuttle vector.

The order of the major features in this plasmid is: pMB1 ori - ampR - pRSF0885 rep - cmlR.

Temperature: 37.0°C