| Vector |
Construct size (kb): 5.0 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: lafmid BA
Intact vector size: 3.153
Type of vector: phagemid
Vector end: HindIII
Vector end: NotI
Cloning sites: HindIII, BamHI, NotI, EcoRI
Polylinker sites: HindIII, BamHI, NotI, EcoRI
Other unique sites: ClaI, XmnI, ScaI, ApaLI
Construction: pGEM
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, <-, 100-556
insert detection: lacZ', <-, 594-1119
primer site: M13 universal, ->
MCS: EcoRI/NotI/BamHI/HindIII, ->, 1061-1098
primer site: M13 reverse, <-
promoter: lac, <-, 1164-1192
replicon: pMB1, <-, 1518-1518
marker(s): ampR, <-, 2276-3136
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: CD36L1
Other gene symbols: CLA-1
Gene name: CD36 antigen (collagen type I receptor, thrombospondin
receptor) - like 1
Contains complete coding sequence?: N
Tissue: infant brain, 3 months old (from Bento Soares)
Type of DNA: cDNA
Insert 5' end: HindIII
Insert 3' end: NotI
Insert size (kb): 2.0
Cross references: DNA Seq. Acc.: T09272; EST: EST07165; Primer: M13-21; THC
id: THC124116
DNA Seq. Acc.: T09273; EST: EST07166; Primer: M13 Reverse;
THC id: THC124116
DNA Seq. Acc.: Z22555
Nucleotides 1-365 of the insert correspond to
nucleotides 570-935 of Z22555 with 99% identity.
DNA Seq. Acc.: Z22555; EST: EST07165
Nucleotides 303-215, 214-2 of the insert correspond to
nucleotides 2252-2340, 2340-2552 of Z22555 with 98% identity. Insert lengths(kb): 2.0 Tissue: infant brain, 3 months old (from Bento Soares) Gene product: scavenger receptor class B type I( CD36 antigen (collagen type I receptor, thrombospondin receptor)-like 1, scavenger receptor class B type 1) [CLA-1] |
| Comments |
Restriction digests of the clone give the following sizes (kb): HindIII/NotI--3.0, 2.0; EcoRI--3.5, 1.5. Verified to contain EST07165 by single-pass sequencing. Based on the alignment with GenBank Z22555 (2566 nt, CDS 70-1599), this clone appears to contain part of the coding sequence and the 3' untranslated region. The cDNAs (>95%) are cloned directionally with the 5' end adjacent to the M13 reverse primer and HindIII. |
| References |
Adams MD, et al. Rapid cDNA sequencing (expressed sequence tags) from a directionally cloned human infant brain cDNA library. Nat. Genet. 4: 373-380, 1993. PubMed: 8401585
Mark D Adams, personal communication
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