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Construct size (kb): 52.0

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pWE15
Intact vector size: 8.200
Type of vector: cosmid
Vector end: BamHI
Vector end: BamHI
Cloning sites: BamHI
Polylinker sites: EcoRI NotI BamHI NotI EcoRI
Host range: vertebrate cells; Escherichia coli
Features (with orientation and position when available):
marker(s): ampR, kanR, G418R
promoter: T3, T7
replicon: pMB1
Cross references: DNA Seq. Acc.: X65279

DNA: genomic

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: SCN4A
Gene name: sodium channel, voltage-gated, type IV, alpha polypeptide
Contains complete coding sequence?: N
Chromosome: 17; Localization: 17 q23.1-q25.3
Tissue: lymphoblast
Type of DNA: genomic
Insert end: BamHI
Insert end: BamHI
Insert size (kb): 44.0
Cross references: DNA Seq. Acc.: L01971
DNA Seq. Acc.: L01972
DNA Seq. Acc.: L01973
DNA Seq. Acc.: L01974
DNA Seq. Acc.: L01975
DNA Seq. Acc.: L01976
DNA Seq. Acc.: L01977
DNA Seq. Acc.: L01978
DNA Seq. Acc.: L01979
DNA Seq. Acc.: L01980
DNA Seq. Acc.: L01981
DNA Seq. Acc.: L01982
DNA Seq. Acc.: L01983

Insert lengths(kb): 44.0

Tissue: lymphoblast

Gene product: sodium channel, voltage-gated, type IV, alpha polypeptide [SCN4A]

Restriction digests of the clone give the following sizes (kb): BamHI--13.0, 10.0, 6.6, 4.8, 4.0, 3.4 (doublet), 0.6; BglII--13.5, 7.0 (doublet), 5.1, 4.2, 3.1, 1.7, 1.6, 1.45; EcoRI--17.0, 9.2, 9.0, 5.9, 2.9, 1.1;

HindIII--17.5, 9.4, 5.8, 3.5, 3.3, 2.95, 0.45; SstI--11.5 (doublet), 4.9, 3.3, 2.95, 2.4 (doublet), 1.25, 1.1, 0.80, 0.62, 0.45, 0.30.

IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.

Includes exons 12 - 24, with exon 24 (and thus the 3' untranslated region) being complete. This corresponds to domains II, III and IV of the protein product.

Additional GenBank/EMBL accession numbers: L01971-L01982.