| Insert |
DNA: genomic Insert lengths(kb): 40.0 Gene product: DNA Segment, single copy [D5S85] Alleles: A1, A2, B1, B2, C1, C2 |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--8.5, 5.4, 4.3, 4.0, 2.4, 1.7, 1.15; HindIII--11, 6.2, 5.7, 4.9, 3.6, 1.45; BglII--10, 8.4, 4.2, 2.9; SalI--27, 8.4; SfiI--27, 8.4. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. The BclI RFLP is in linkage disequilibrium with the TaqI RFLP. |
