IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.
Broad host range cosmid vector which can be selected and replicate in Escherichia coli, Saccharomyces cerevisiae, and Vibrio parahaemolyticus.
Media
ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline
Growth Conditions
Temperature: 30.0°C
References
Datta AR, et al. Shuttle cloning vectors for the marine bacterium Vibrio parahaemolyticus. J. Bacteriol. 160: 808-811, 1984. PubMed: 6094492
