| 產(chǎn)品名稱(chēng) |
mKV1.3-EC |
| 商品貨號(hào) |
B207180 |
| Designations |
mKV1.3-EC |
| GenBank Number |
M30441
|
| Species |
Mus musculus, mouse |
| Depositors |
G Chandy |
| Applications |
construct to generate mRNA that can be expressed in Xenopus oocytes potassium voltage-gated channel, shaker-related subfamily, member 3 potassium voltage-gated channel, shaker related subfamily, type n |
| Vector |
Construct size (kb): 5.0 |
| Insert |
DNA: genomic Insert lengths(kb): 1.76800000667572 Gene product: potassium voltage-gated channel, shaker-related subfamily, member 3(potassium voltage-gated channel, shaker related subfamily, type n) [Kcna3] Target Gene: potassium voltage-gated channel, shaker-related subfamily, member 3 |
| Insert Size (kb) |
1.768 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): BglII/BstEII--3.2, 1.8; BstEII--5.0; BglII--5.0; BamHI--5.0; EcoRI--5.0. The insert contains the following restriction sites (approximate kb from the 5' end): BglII--0.00; BanI--0.02; NcoI--0.44; PstI--1.02; SacII--0.15. For in vitro transcription, linearize with EcoRI and use SP6 RNA polymerase. |
| Classification |
Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
| References |
Grissmer S, et al. Expression and chromosomal localization of a lymphocyte K+ channel gene. Proc. Natl. Acad. Sci. USA 87: 9411-9415, 1990. PubMed: 2251283
Krieg PA, Melton DA. Functional messenger RNAs are produced by SP6 in vitro transcription of cloned cDNAs. Nucleic Acids Res. 12: 7057-7070, 1984. PubMed: 6207484
George Chandy, personal communication
|
