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Construct size (kb): 6.498000144958496

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pGEM-7Zf(-)
Intact vector size: 3.000
Type of vector: phagemid
Vector end: EcoRI
Vector end: KpnI
Cloning sites: ApaI AatII SphI XbaI XhoI EcoRI KpnI SmaI Csp45I ClaI
HindIII BamHI SacI BstXI NsiI
Polylinker sites: ApaI AatII SphI XbaI XhoI EcoRI KpnI SmaI Csp45I ClaI
HindIII BamHI SacI BstXI NsiI
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ'
marker(s): ampR
promoter: lac, SP6, T7
replicon: pMB1, f1
Cross references:

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: IREB1
Other gene symbols: IRE1; FRP*; IRF*
Gene name: iron-responsive element binding protein 1
Contains complete coding sequence?: Y
Chromosome: 9; Localization: 9
Strain: fibroblast cell line EK4
Tissue: placenta
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 3.498
Cross references: DNA Seq. Acc.: Z11559

Insert lengths(kb): 3.497999906539917

Tissue: placenta

Gene product: aconitase(iron-responsive element binding protein 1) [FRP*]

Restriction digests of the clone give the following sizes (kb): XbaI/ClaI--3.55, 3.0; EcoRI--3.1, 1.35, 1.3, 0.7; PstI--6.55; BamHI--3.4, 3.15; HindIII--6.2, 0.5.

The insert contains the following restriction sites (bp from the 5' end): BamHI--143; BglI--712, 2262, 2493, 2854; EcoRI--1368, 2015, 2220; HindIII--3040; KpnI--2384; PstI--364; PvuII--2154, 3104.

A probe derived from sequence between nt 44 and 344 detects a single genomic EcoRI fragment of approximately 4.3 kb in human placental DNA.

Contains 107 bp of 5' untranslated region, the complete coding sequence and 720 bp of 3' untranslated region.

Detects a single mRNA of approximately 4.0 kb in the fibroblast cell line EK4.

Constructed from clones isolated from placenta and fibroblast libraries.