| 產(chǎn)品名稱 |
pSODalpha2 |
| 商品貨號(hào) |
B207241 |
| Designations |
pSODalpha2 |
| Species |
Homo sapiens, human |
| Depositors |
Bio-Technology General Corporation, JP White, Bio-Technology General Corporation |
| Vector |
Construct size (kb): 2.880000114440918 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBRM
Intact vector size: 2.000
Type of vector: plasmid
Vector end: BamHI
Vector end: BamHI
Cloning sites: BamHI EcoRI
Polylinker sites:
Construction: pBR322
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): ampR
replicon: pMB1
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: human
Gene symbol: SOD1
Genomic copy number: unique
Gene name: superoxide dismutase 1, soluble
Contains complete coding sequence?: U
Chromosome: 21; Localization: 21 q22.1
Type of DNA: cDNA
Insert end: HindIII
Insert end: NdeI
Insert size (kb): 0.88
Cross references: Insert lengths(kb): 0.8799999952316284 Gene product: superoxide dismutase 1, soluble(superoxide dismutase, Cu-Zn) [SOD1] Alleles: B1, B2 |
| Insert Size (kb) |
0.880 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--2.0, 0.88; HindIII--uncut; EcoRI--2.6, 0.30. This should not be grown at temperatures exceeding 30C. |
| References |
Lieman-Hurwitz J, et al. Human cytoplasmic superoxide dismutase cDNA clone: a probe for studying the molecular biology of Down syndrome. Proc. Natl. Acad. Sci. USA 79: 2808-2811, 1982. PubMed: 6211674
Hartman JR, et al. Method for producing enzymatically active eucaryotic SOD in bacteria. US Patent 4,742,004 dated May 3 1988
Jacob C Hartman, personal communication
|
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
