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Construct size (kb): 3.86899995803833

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pGEM-7Zf
Intact vector size: 3.000
Type of vector: phagemid
Vector end: EcoRI
Vector end: XbaI
Cloning sites: ApaI AatII SphI XbaI XhoI EcoRI KpnI SmaI Csp45I ClaI
HindIII BamHI SacI BstXI NsiI
Polylinker sites: ApaI AatII SphI XbaI XhoI EcoRI KpnI SmaI Csp45I ClaI
HindIII BamHI SacI BstXI NsiI
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
insert detection: lacZ'
marker(s): ampR
promoter: lac, SP6, T7
replicon: pMB1, f1
terminator: none
Cross references:

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: ADRB3
Gene name: adrenergic, beta-3-, receptor
Contains complete coding sequence?: U
Localization: 8 p12-p11.1
Tissue: SK-N-MC cells
Type of DNA: cDNA
Insert 5' end: EcoRI
Insert 3' end: XbaI
Insert size (kb): 0.869
Cross references: DNA Seq. Acc.: S53291

Insert lengths(kb): 0.8690000176429749

Tissue: SK-N-MC cells

Gene product: adrenergic, beta-3-, receptor [ADRB3]

Restriction digests of the clone give the following sizes (kb): BamHI--2.9, 0.5, 0.4; BglI--1.54, 1.32, 0.9, 0.04; EcoRI--3.87; EcoRI/XbaI--3.0, 0.87; NcoI--3.44, 0.43.

The insert contains the following restriction sites (approximate kb from the 5' end): BamHI--0.36; BglI--0.07, 0.11; NcoI--0.29, 0.72; SacI--0.55, 0.78.

Corresponds to nt 1137-2006 of GenBank accession X70811, spanning a region that contains an intron in the genomic sequence.

Constructed after amplification from cDNA with these primer/adapters: 5'-TGCGAATTCTGCCTTCAACCCGCTC-3'; 5'-ACTATAGGGTCTAGAGGATCCG-3'. The product was digested with EcoRI + XbaI.

Transcription from the SP6 promoter gives the sense strand.