| Insert |
DNA: cDNA Insert lengths(kb): 2.053999900817871 Tissue: fibroblast, PMA-treated Gene product: matrix metalloproteinase 1 (interstitial collagenase)(collagenase, epidermolysis bullosa, dystrophic, (autosomal recessive), collagenase, fibroblast, collagenase, interstitial) [CLG] |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--4.9, 0.3; BamHI--3.8, 1.3; PstI--5.1; HindIII--5.1; AvaI--5.1; HindIII/AccI--3.1, 2.0; HindIII/BglII--5.1; HindIII/SmaI--3.1, 2.0. Contains the complete coding sequence. Transcription from the SP6 promoter yields RNA complementary to the mRNA. If the first nucleotide after the dC tail is counted as 1, the poly(A) tract of 75 bp begins at nt 1969. The insert contains XbaI, EcoRI, XmnI, EcoRV, SacI, BamHI, PstI, XbaI, BglII, and HindII sites. The first ATG (nt 67-69) opens a reading frame of 469 aa. The 5' untranslated region contains a CCATC ribosome binding site (nt 26-30). The sequence TTATTTAT (nt 1597-1604) in the 3' untranslated region is also found in mRNA for TNF, IL1, fibronectin, CSF, and interferons. Enzyme(s) not detecting polymorphism: TaqI, MspI, BglII, RsaI, BamHI, XmnI, SacI, PvuII, EcoRV. |
| References |
Whitham SE, et al. Comparison of human stromelysin and collagenase by cloning and sequence analysis. Biochem. J. 240: 913-916, 1986. PubMed: 3030290
Angel P, et al. 12-O-tetradecanoyl-phorbol-13-acetate induction of the human collagenase gene is mediated by an inducible enhancer element located in the 5'-flanking region. Mol. Cell. Biol. 7: 2256-2266, 1987. PubMed: 3037355
Spurr NK, et al. Restriction fragment length polymorphism analysis and assignment of the metalloproteinases stromelysin and collagenase to the long arm of chromosome 11. Genomics 2: 119-127, 1988. PubMed: 2900807
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