| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--15.0, 8.5, 7.5, 2.7, 1.85, 1.8, 1.05, 1.0, 0.55; BamHI/EcoRI--9.5, 7.5, 5.8, 3.0, 2.5, 1.85, 1.8, 1.6, 1.5, 1.05, 1.0, 0.75, 0.55, 0.55; HindIII/EcoRI--12.5, 4.55, 3.6, 3.0, 2.9, 2.7, 2.5, 1.75, 1.7, 1.2, 1.08, 1.08, 1.0, 0.7, 0.55; KpnI/EcoRI--15.0, 8.5, 7.5, 2.7, 1.85, 1.8, 1.05, 1.0, 0.55; SalI/EcoRI--15.0, 7.5, 5.0, 2.7, 2.5, 2.5, 1.85, 1.8, 1.05, 1.0, 0.55. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. Contains the complete gene (4 exons). |
| References |
Lau YF, Kan YW. Versatile cosmid vectors for the isolation, expression, and rescue of gene sequences: studies with the human alpha-globin cluster. Proc. Natl. Acad. Sci. USA 80: 5225-5229, 1983. PubMed: 6310566
Gitt MA, et al. Isolation and expression of a gene encoding L-14-II, a new human soluble lactose-binding lectin. J. Biol. Chem. 267: 10601-10606, 1992. PubMed: 1375225
Gitt MA, Barondes SH. Genomic sequence and organization of two members of a human lectin gene family. Biochemistry 30: 82-89, 1991. PubMed: 1988031
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