| 產品名稱 |
hp35 |
| 商品貨號 |
B207726 |
| Designations |
hp35 |
| GenBank Number |
M38443
M65271
|
| Species |
Homo sapiens, human |
| Depositors |
U Gubler |
| Applications |
The cDNA contains two in-frame Met codons: one at position 100-102 and the second one at position 202-204. Only the second Met codon was used for expression of bioactive IL-12 in mammalian cells by the depositor. |
| Vector |
Construct size (kb): 4.064000129699707 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK+
Intact vector size: 2.964
Type of vector: phagemid
Vector end: EcoRV
Vector end: EcoRV
Cloning sites: KpnI Eco0109I ApaI DraII XhoI SalI HincII AccI ClaI Bsp106I
HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI
SacII BstXI SacI
Polylinker sites: KpnI Eco0109I ApaI DraII XhoI SalI HincII AccI ClaI Bsp106I
HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI
SacII BstXI SacI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
insert detection: lacZ'
marker(s): ampR
promoter: lac, T3, T7
replicon: pMB1, f1
terminator: none
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: IL12A
Other gene symbols: IL-12 p35
Gene name: interleukin 12A (natural killer cell stimulatory factor 1,
cytotoxic lymphocyte maturation factor 1, p35
Contains complete coding sequence?: Y
Chromosome: 3; Localization: 3 p25-p26.2
Type of DNA: cDNA
Insert 5' end: EcoRV
Insert 3' end: EcoRV
Insert size (kb): 1.1
Cross references: DNA Seq. Acc.: M38443
Nucleotides 33 - 1058 of the insert correspond to
nucleotides 1 - 1026 of M38443.
DNA Seq. Acc.: M65271
Nucleotides 33 - 1058 of the insert correspond to
nucleotides 1 - 1026 of M65271. Insert lengths(kb): 1.100000023841858 Gene product: interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35 [IL12A] |
| Insert Size (kb) |
1.100 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): NotI/SalI--2.9, 1.2; HindIII--3.4, 0.65; EcoRV--4.1. The insert contains the following restriction sites (approximate kb from the 5' end): EcoRV--0.42; HindIII--0.63; BglII--0.66. The cDNA contains two in-frame Met codons: one at position 100-102 and the second one at position 202-204. Only the second Met codon was used for expression of bioactive IL-12 in mammalian cells by the depositor. The insert, p35 cDNA including 5' and 3' noncoding regions,was PCR amplified out of a lambda phage clone and ligated into the EcoRV site of pBluescript SK+. |
| References |
Gubler U, et al. Coexpression of two distinct genes is required to generate secreted bioactive cytotoxic lymphocyte maturation factor. Proc. Natl. Acad. Sci. USA 88: 4143-4147, 1991. PubMed: 1674604
|
