| Comments |
Restriction digests of the clone give the following sizes (kb): HindIII--10.0, 9.4, 5.6, 4.2, 3.5, 3.0, 1.9, 1.7, 1.5, 1.3, 1.2, 0.8, 0.5; SalI-->23.1, 2.4; AvaI--11.0, 8.8, 5.6, 4.4, 3.3, 2.1 (doublet), 1.9, 1.5, 0.8, 0.7, 0.3; NotI-->23.1, 8.0; BamHI--9.4, 8.4, 6.0, 4.3, 3.8, 3.5, 2.6, 1.9, 1.8, 1.4 (doublet); EcoRI--8.2, 7.8, 6.5, 5.4, 5.2, 4.7, 2.6, 2.2, 1.6, 0.4. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. Enzyme(s) not detecting polymorphism: MspI, PstI. |
