| Comments |
Restriction digests of the clone give the following sizes (kb): HindIII--10.0, 6.2, 4.6, 3.5 (doublet), 2.1, 1.15, 0.95; BglII--8.4, 7.0, 5.5, 4.9, 3.8, 1.4, 1.15; SalI--23, 8.4; SfiI--19, 8.3, 5.4; EcoRI--8.2, 4.8, 2.45, 1.8, 1.7, 1.1, 0.82, 0.7, 0.6, 0.45 + smaller. Codominant segregation was observed in 60 families. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. Enzyme(s) not detecting polymorphism: PstI, PvuII, MspI. |
| References |
Weiffenbach B, et al. A genetic linkage map of human chromosome 5 with 60 RFLP loci. Genomics 10: 173-185, 1991. PubMed: 1675192
Raymond L White, personal communication
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