ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Comments
The insert contains the following sites separated by (approximate kb): BamHI- 0.24- PstI- 0.45- BamHI- 1.0- BamHI- 0.63- XhoI- 0.29- PstI- 2.3- XbaI- 1.0- BamHI- 0.6- XbaI- 1.1- XbaI- 2.9- BamHI- 0.25- BamHI.
Restriction digests of the clone give the following sizes (kb): EcoRI/SalI--11, 3.7, 3.3, 2.2, 1.05, 0.94; KpnI--uncut; XhoI--15, 7; XbaI--16, 4.2, 1.45; PvuII--17, 5.1.
The insert includes a continuous open reading frame of 2682 nt encoding a protein of 894 amino acids and a molecular weight of 102 kDa.
The probe, pG59/T1, has been deposited in Escherichia coli RR1 as ATCC 37668 and in Saccharomyces cerevisiae C151/L1 as ATCC 66312.
Tzagoloff A, et al. Homology of yeast mitochondrial leucyl-tRNA synthetase and isoleucyl- and methionyl-tRNA synthetases of Escherichia coli. J. Biol. Chem. 263: 850-856, 1988. PubMed: 2826465
