Plasmid is temperature inducible to produce infectious phage particles.
Designed to identify genes by complementation of E. coli host mutations in hosts producing lambda cI protein. Accepts inserts of 6-24 kb.
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 30.0°C
References
Elledge SJ, Walker GC. Phasmid vectors for identification of genes by complementation of Escherichia coli mutants. J. Bacteriol. 162: 777-783, 1985. PubMed: 2985547
