| 產(chǎn)品名稱 | TIME-GFP |
|---|---|
| 商品貨號 | B208644 |
| Organism | Homo sapiens, human |
| Tissue | foreskin |
| Cell Type | microvascular endothelial cell |
| Product Format | frozen |
| Morphology | endothelial |
| Culture Properties | adherent |
| Biosafety Level | 2 [Cells contain SV40 viral DNA sequences]
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | normal |
| Age | neonatal |
| Gender | male |
| Applications | Both TIME-GFP (ATCC CRL-4045) and its parental cell line TIME (ATCC CRL-4025) exhibit normal diploid karyotype, extended lifespan in culture and endothelial characteristics that make them an ideal model for investigating many aspects of endothelial cell biology. TIME-GFP (ATCC CRL-4045) retains angiogenic potential and stably expresses green fluorescence protein (GFP) for at least 12 passages. The GFP-expressing cells migrate and coalesce into networks of vessel-like structure within 8 hours after being plated onto basement membrane gel (CellMatrix, ATCC ACS-3035). The stable expression of GFP in these cells enables detection and analysis of the fragile endothelial structures to occur without post-assay fixation and/or staining. |
| Shipping Information | FZ |
| Storage Conditions | liquid nitrogen vapor phase |
| Karyotype | This is a diploid cell line of male origin with a modal chromosome number of 46 and a low rate of polyploidy. The line shows some karyotypic instability at later passages. |
| Images | 
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| Derivation | TIME-GFP (ATCC CRL-4045) is derived by transfecting TIME (ATCC CRL-4025) cells with linearized pWE2-EmGFP plasmid, which constitutively expresses the Green Fluorescence Protein (GFP) driven by a CMV promoter. A stable GFP-expressing clone is selected to establish the TIME-GFP cell line. |
| Clinical Data | male neonatal |
| Antigen Expression | Positive for CD31 and capable of uptaking Low Density Lipoprotein (LDL). Express Green Fluorescence Protein (GFP) constitutively |
| Complete Growth Medium | The base medium for this cell line is Vascular Cell Basal Medium (ATCC PCS-100-030), supplemented with Microvascular Endothelial Cell Growth Kit-BBE (ATCC PCS-110-040) OR Microvascular Endothelial Cell Growth Kit-VEGF (ATCC PCS-110-041). Add Blasticidin (Life Technologies Cat. No. A11139-03) at a final concentration of 12.5 ug/mL and G418 (Life Technologies Cat. No. 11811-023) at a final concentration of 200 ug/mL. Note: Do not filter complete medium.
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| Subculturing | Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation solutions for culture vessels of other sizes. Subculture when the culture is about 90% confluent.
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| Cryopreservation | 90% FBS, 10% DMSO |
| Culture Conditions | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C |
| STR Profile | D5S818: 11 D13S317: 9, 11 D7S820: 8, 9 ; D16S539: 9, 12 vWA: 16, 18 Amelogenin: X, Y TPOX: 8 CSF1PO: 11, 12 TH01: 6, 7 |
| Population Doubling Level (PDL) | As part of our quality control, we have tested this cell line for its ability to grow for a minimum of 15 population doublings after recovery from cryopreservation. We have also compared its karyotype, telomerase expression level, growth rate, morphology and tissue-specific markers when first recovered from cryopreservation with that of cells at 10+ population doublings to ensure that there is no change in these parameters and that the cells are capable of extended proliferation. |
| Name of Depositor | ATCC |
| 梅經(jīng)理 | 17280875617 | 1438578920 |
| 胡經(jīng)理 | 13345964880 | 2438244627 |
| 周經(jīng)理 | 17757487661 | 1296385441 |
| 于經(jīng)理 | 18067160830 | 2088210172 |
| 沈經(jīng)理 | 19548299266 | 2662369050 |
| 李經(jīng)理 | 13626845108 | 972239479 |
