| 產品名稱 |
pMev |
| 商品貨號 |
B208686 |
| Designations |
pMev |
| GenBank Number |
M97382
|
| Species |
Cricetulus griseus, hamster, Chinese |
| Depositors |
JL Goldstein |
| Vector |
Construct size (kb): 9.300000190734863 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: Rc/CMV7S
Intact vector size: 6.000
Type of vector: plasmid
Vector end: SalI
Vector end: NotI
Cloning sites:
Polylinker sites:
Host range: vertebrate cells; Escherichia coli
Features (with orientation and position when available):
marker(s): ampR, neoR
promoter: CMV
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: hamster, Chinese
Gene symbol: MEVT*
Gene name: mevalonate transporter
Contains complete coding sequence?: U
Tissue: MET-18b-2 cell line
Type of DNA: cDNA
Insert 5' end: SalI
Insert 3' end: NotI
Insert size (kb): 3.345
Cross references: DNA Seq. Acc.: M97382 Insert lengths(kb): 3.34500002861023 Tissue: MET-18b-2 cell line Gene product: mevalonate transporter(monocarboxylate transporter 1) [MEVT*] |
| Insert Size (kb) |
3.345 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): NotI/SalI--6.0, 3.3; SalI--9.3; NotI--9.3; EcoRI--6.1, 2.2; XhoI--8.0, 1.0, 0.3. The insert contains the following restriction sites (approximate kb from the 5 ' end): SmaI--0.05, 2.9; EcoRI--2.1; XhoI--2.3. Transcription of the insert is regulated by the CMV promoter, and transcription of neoR is regulated by the SV40 early promoter. The ends of the insert were derived from linker adapters. |
| References |
Otto JC, Casey PJ. The hepatitis delta virus large antigen is farnesylated both in vitro and in animal cells. J. Biol. Chem. 271: 4569-4572, 1996. PubMed: 8617711
Kim CM, et al. cDNA cloning of MEV, a mutant protein that facilitates cellular uptake of mevalonate, and identification of the point mutation responsible for its gain of function. J. Biol. Chem. 267: 23113-23121, 1992. PubMed: 1429658
Joseph L Goldstein, personal communication
|
