Gene product: Fc gamma low affinity receptor III, alpha subunit (CD16) [CD16]
Insert Size (kb)
1.341
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--3.25, 1.4; BamHI--3.5, 1.15; PvuII--2.9, 1.1, 0.65; SacI--3.6, 1.05.
The insert contains the following sites (in bp from the 5' end): BamHI--195; NsiI--636; PvuII--499; SacI--290; SmaI--1013.
The insert contains 49 bp 5' untranslated, a complete open reading frame (49-850) and 491 bp 3' untranslated sequence. The extracellular domain is encoded from 143-694, the transmembrane region from 695-850, and the cytoplasmic domain from 783-850.
The insert is oriented in the + strand direction relative to the universal primer.
References
Zeger DL, et al. Characterization and expression of an Fcgamma receptor cDNA cloned from rat natural killer cells. Proc. Natl. Acad. Sci. USA 87: 3425-3429, 1990. PubMed: 1692135
