Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format
frozen 200 μL per vial
Storage Conditions
?-70°C or colder
Comments
ATCC® VR-1540P™ is a purified virus product suspended in 200 µL of Hank's Balanced Salt Solution with Ca2+, Mg2+ and 25 mM HEPES Buffer. ATCC® VR-1540™ is prepared from original isolate deposited as ATCC® VR-1302™, which was grown in presence of neutralizing serum to remove contaminating adenovirus type 1.
Effect on Host
CPE, syncytial formation, rounding, and sloughing
Recommended Host
HeLa (ATCC® CCL-2™)
Growth Conditions
Temperature: 37°C
Recommendations for Infection: Plate cells 24 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an MOI 0.1. Adsorb for 1 hour at 37°C in a humidified 5% CO2 atmosphere, rocking every 15 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 3 to 10 days
Name of Depositor
ATCC, L Potash
Source
Lower respiratory tract of infant with bronchiolitis and bronchopneumonia, Melbourne, Australia, 1961
References
Cameron R, et al. Identification of contaminating adenovirus type 1 in the ATCC reference strain of respiratory syncytial virus A2 (VR-1302). Virus Res. 92: 151-156, 2003. PubMed: 12686423
Lewis FA, et al. A syncytial virus associated with epidemic disease of the lower respiratory tract in infants and young children. Med. J. Aust. 2: 932-933, 1961.
