| Vector |
Intact vector size: 6.868
Type of vector: phagemid
Cloning sites: SacI SacII EagI NotI SpeI BamHI SmaI PstI EcoRI ClaI SalI XhoI ApaI KpnI
Polylinker sites: SacI BstXI SacII EagI NotI XbaI SpeI BamHI SmaI PstI EcoRI EcoRV HindIII ClaI SalI XhoI ApaI KpnI
Construction: pRS402, pRS421
Host range: Saccharomyces cerevisiae; Escherichia coli
Features (with orientation and position when available):
marker(s): ADE2, →, 575-2290
replicon: f1, ←, 2495-2951
promoter for in vitro transcription: T7, →, 3121-3140
MCS: KpnI...SacI, →, 3148-3250
promoter for in vitro transcription: T3, ←, 3267-3286
coding sequence: lacZ', ←, 2952-3311
promoter: lac, ←, 3356-3384
replicon: pMB1, 3710-3710
marker(s): ampR, ←, 4468-5328
replicon: 2 micron, 5460-6801
Vector: pRS422 (phagemid)
Promoters: Promoter for in vitro transcription T7
Construction: pRS402, pRS421
Marker(s): ampR,ADE2
Construct size (kb): 6.868
Features: marker(s): ADE2
marker(s): ampR
promoter: lac
promoter for in vitro transcription: T3
promoter for in vitro transcription: T7
replicon: 2 micron
replicon: f1
replicon: pMB1
MCS: KpnI...SacI
coding sequence: lacZ' |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--6.8; BglII--4.6, 2.2; EcoRI--6.8. ade2 phenotype produces red colonies when grown on adenine containing media. This vector is useful for gene knockout experiments in hosts with a non-revertable ade2 auxotrophic marker gene mutation. |
