| 產品名稱 |
pGRN33 |
| 商品貨號 |
B209311 |
| Designations |
pGRN33 |
| GenBank Number |
U86046
|
| Species |
Homo sapiens, human |
| Depositors |
Geron Corporation, B Villeponteau, Geron Corporation |
| Vector |
Construct size (kb): 5.5 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript
Intact vector size: 2.960
Type of vector: phagemid
Cloning sites:
Polylinker sites:
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
insert detection: lacZ'
marker(s): ampR
promoter: lac, T3, T7
replicon: pMB1, f1
terminator: none
Cross references: |
| Insert |
DNA: genomic DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: hTR*
Gene name: telomerase RNA
Contains complete coding sequence?: Y
Tissue: lung fibroblast cell line WI-38
Type of DNA: genomic
Insert end: HindIII
Insert end: SacI
Insert size (kb): 2.5
Cross references: DNA Seq. Acc.: U86046
Nucleotides ??? - ??? of the insert correspond to
nucleotides 1-451 of U86046. Insert lengths(kb): 2.5 Tissue: lung fibroblast cell line WI-38 Gene product: telomerase RNA [hTR*] |
| Insert Size (kb) |
2.5 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: DNA supplied by depositor (frozen) |
| Comments |
Restriction digests of the clone give the following sizes (kb): HindIII--6.9; HindIII+SacI--4.4, 2.5. Digestion with EcoRI yields the following fragment sizes (approximate kb): 5.5 and 1.2. |
| References |
Villeponteau B, et al. Mammalian telomerase. US Patent 5,583,016 dated Dec 10 1996
|
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
