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Construct size (kb): 4.300000190734863

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pJFE14
Intact vector size: 3.500
Type of vector: plasmid
Vector end: BstXI
Vector end: NotI
Cloning sites: BstXI NotI SacII SfiI EcoRI
Polylinker sites: BstXI NotI SacII SfiI EcoRI
Construction: pcDL-SRalpha296
Host range: mammalian cells; Escherichia coli
Features (with orientation and position when available):
replicon: SV40
promoter for expression: SV40 early region
enhancer: HTLV-1 R-U5 segment of LTR
MCS: BstXI...EcoRI
terminator: SV40 late region polyadenylation signal
replicon: pMB1
marker(s): ampR, <-
Cross references:

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: CD94
Gene name: CD94 antigen (NK/T-cell C-type lectin receptor)
ORF's seq. position: GDB probe: GDB:696221 ()
Contains complete coding sequence?: Y
Chromosome: 12; Localization: 12 p13
Tissue: polyclonal NK cell line
Type of DNA: cDNA
Insert 5' end: Modification: BstXI linker
Insert 3' end: Modification: NotI linker
Insert size (kb): 0.8
Cross references: DNA Seq. Acc.: U30610
Nucleotides 1-834 of the insert correspond to
nucleotides 1-834 of U30610.

Insert lengths(kb): 0.800000011920929

Tissue: polyclonal NK cell line

Gene product: CD94 antigen (NK/T-cell C-type lectin receptor) [CD94]

Restriction digests of the clone give the following sizes (kb): BstXI--4.2; BstXI/NotI--3.4, 0.84; NotI--4.2.

The insert contains the following restriction sites (approximate kb from the 5' end): KpnI--0.28; SacI--0.41.