| 產(chǎn)品名稱 |
Human rhinovirus 39 |
| 商品貨號 |
B209430 |
| Classification |
Picornaviridae, Enterovirus, Rhinovirus A |
| Deposited As |
Rhinovirus 39 |
| Agent |
Human rhinovirus 39 |
| Strain |
209 |
| Applications |
Respiratory research |
| Biosafety Level |
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format |
frozen 1 mL per vial |
| Storage Conditions |
-70°C or colder |
| Comments |
Growth not inhibited by IUDR. Stabilized by 1 M MgCl2. Does not hemagglutinate rat, rhesus monkey, or human blood type O. Non-pathogenic for suckling mice. |
| Effect on Host |
CPE, refractile rounding and sloughing |
| Recommended Host |
H1-HeLa (ATCC® CRL-1958™) |
| Growth Conditions |
Temperature: 33°C
Recommendations for Infection: Plate cells 24 hours prior to infection and infect when cultures are 80% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 33°C in a humidified atmosphere while rocking continuously. End adsorption by adding virus growth medium.
Incubation: 1-7 days while rocking continuously |
| Name of Depositor |
MA Mufson, National Institute of Allergy and Infectious Diseases; RM Conant, VV Hamparian, The Ohio State University |
| Source |
Nasal washing from an adult female, North Carolina |
| References |
Mufson MA, et al. A description of six new Rhinoviruses of human origin. Am. J. Epidemiol. 81: 32-43, 1965. PubMed: 14246079
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