Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Comments
The insert contains the following sites separated by (kb) (approximate): SphI- 1.4- SacI- 0.6- HindIII- 1.6- KpnI- HindIII- 0.8- SphI- 1.2- SphI.
The smallest subclone capable of complementing msf1-1, msf1-3, and msf1-4 mutations contained a 2.2 kb SacI/KpnI fragment.
MSF1 is a nuclear gene encoding the alpha subunit of the mitochondrial phenylalanyl-tRNA synthetase.
Carries plasmid pG120/T1 with wild-type gene
The probe, pG120/T1, has been deposited in Escherichia coli RR1 as ATCC 37664 and in Saccharomyces cerevisiae C303/L2 as ATCC 66317.
Koerner TJ, et al. Isolation and characterization of the yeast gene coding for the alpha subunit of mitochondrial phenylalanyl-tRNA synthetase. J. Biol. Chem. 262: 3690-3696, 1987. PubMed: 3029120
