ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Comments
Restriction digests of the clone give the following sizes (kb): HindIII--2.9, 1.8; BamHI--4.8; EcoRI--3.7, 1.15; PstI--3.4, 1.45; PvuII--2.6, 2.2; XbaI--4.8.
Restriction digests of the plasmid give the following sizes (kb): HindIII--2.96, 1.9; BamHI--4.8; EcoRI--3.7, 1.1; PstI--3.3, 1.5; SspI--2.4, 1.3, 1.0.
Contains the exon encoding the first part of the bait region (homologous to exon 17 of the rat gene), as well as portions of flanking introns.
References
Poller W, et al. Cloning of the human alpha2-macroglobulin gene and detection of mutations in two functional domains: the bait region and the thiolester site. Hum. Genet. 88: 313-319, 1992. PubMed: 1370808
