| 產(chǎn)品名稱 | Human respiratory syncytial virus |
|---|---|
| 商品貨號 | B210557 |
| Classification | Paramyxoviridae, Pneumovirus |
| Agent | Human respiratory syncytial virus |
| Strain | ATCC-2012-11 |
| Biosafety Level | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format | frozen |
| Storage Conditions | -70°C or colder |
| Images |  |
| Comments | This item was isolated in cell culture by three passages in HEp-2 cells This strain belongs to subtype B |
| Effect on Host | CPE, refractile cell rounding and syncytia |
| Recommended Host | HEp-2 (ATCC® CCL-23™) |
| Growth Conditions | Temperature: 37°C
Recommendations for Infection: For best results, infection should be performed on a 70-80% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2), optimized for propagation (e.g. MOI 0.1-0.5 recommended). Wash monolayer with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium. Incubation: 4-6 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer. |
| Name of Depositor | ATCC |
| Source | Specimen from a clinical patient in Virginia, 2012 |
| 梅經(jīng)理 | 17280875617 | 1438578920 |
| 胡經(jīng)理 | 13345964880 | 2438244627 |
| 周經(jīng)理 | 17757487661 | 1296385441 |
| 于經(jīng)理 | 18067160830 | 2088210172 |
| 沈經(jīng)理 | 19548299266 | 2662369050 |
| 李經(jīng)理 | 13626845108 | 972239479 |
