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pGMC
pGMC
規格:
貨期:
編號:B210568
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱 pGMC
商品貨號 B210568
Designations pGMC
GenBank Number

X52259

Species Homo sapiens, human
Depositors ML Lane
Vector
Construct size (kb): 3.160000085830689
Insert
DNA: genomic
Insert lengths(kb): 0.4199999868869782
Tissue: DNA from NIH 3T3 cells transformed by MCF7 DNA
Gene product: sec oncogene [SEC*]
Insert Size (kb) 0.420
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): HincII/AccI--uncut; PvuII--2.35, 0.8; HindIII--3.1; EcoRI--3.1; EcoRI/HindIII--2.6, 0.5.
The insert extends from the PvuII site at position 41 of the published sequence to the AccI site at 462. Coding sequences extend from 21 to 347.
The insert is oriented such that transcription from the SP6 promoter gives the sense strand and from the T7 promoter gives the antisense strand.
References

Lane MA, et al. Nucleotide sequence of a human oncogene active in tumors of secretory epithelium. Nucleic Acids Res. 18: 3068, 1990. PubMed: 2349114

Lane MA, et al. Activation of related transforming genes in mouse and human mammary carcinomas. Proc. Natl. Acad. Sci. USA 78: 5185-5189, 1981. PubMed: 6272293

Mary Ann L Lane, personal communication

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