| Insert |
DNA: genomic DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Genomic copy number: unique
Gene name: gene X
Contains complete coding sequence?: Y
Chromosome: 9; Localization: 9 q34.1
Genome: Homo sapiens
Gene symbol: ABL1
Other gene symbols: ABL
Genomic copy number: unique
Gene name: Abelson murine leukemia viral (v-abl) oncogene homolog 1
Contains complete coding sequence?: Y
Chromosome: 9; Localization: 9 q34.1
Tissue: lung carcinoma
Type of DNA: genomic
Insert end: MboI
Insert end: MboI
Insert size (kb): 35.962
Cross references: DNA Seq. Acc.: U07561
Nucleotides 1-35962 of the insert correspond to
nucleotides 1-35962 of U07561. Insert lengths(kb): 35.96200180053711 Tissue: lung carcinoma Gene product: Abelson murine leukemia viral (v-abl) oncogene homolog 1 [ABL] Alleles: D1, D2, D1, D2, A1, A2, B2, C1, C2, A1, A2, B1, B2, C1, C2, B1 |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI-->23.0; BglII--12.0, 6.6, 6.1, 5.8, 4.6 (doublet), 4.2, 2.8, 0.52; EcoRI--8.0, 7.0, 6.6, 4.9, 4.2 (doublet), 2.65, 2.15 (doublet), 1.55 (doublet); HindIII--14.0 (doublet), 6.1, 2.55 (doublet), 2.3, 2.05, 1.15, 0.96, 0.74, 0.44; SstI--14.0, 10.5, 7.0, 5.8 (doublet), 3.8, 0.92. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. The insert begins 8604 nt upstream of "gene X", includes all 8 exons of that gene (through nt 18583) and continues through exon 1b (29132-29267) of ABL1. A CML breakpoint occurs in the intergenic region. |
| References |
Groffen J, et al. Isolation of human oncogene sequences (v-fes homolog) from a cosmid library. Science 216: 1136-1138, 1982. PubMed: 6281890
Morris CM, et al. Entire ABL gene is joined with 5'-BCR in some patients with philadelphia-positive leukemia. Blood 78: 1078-1084, 1991. PubMed: 1868241
Chissoe SL, et al. Sequence and analysis of the human ABL gene, the BCR gene, and regions involved in the Philadelphia chromosomal translocation. Genomics 27: 67-82, 1995. PubMed: 7665185
Bruce A Roe, personal communication
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