| 產品名稱 |
pPW71B [PW71B] |
| 商品貨號 |
B210756 |
| Designations |
pPW71B [PW71B] |
| Species |
Homo sapiens, human |
| Applications |
contains sequence useful for DNA diagnostics DNA Segment, single copy probes |
| Vector |
Construct size (kb): 3.049999952316284 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pUC19
Intact vector size: 2.686
Type of vector: plasmid
Vector end: SmaI
Vector end: SmaI
Cloning sites: EcoRI SacI KpnI SmaI BamHI XbaI HincII AccI SalI PstI SphI
HindIII
Polylinker sites: EcoRI SacI KpnI SmaI BamHI XbaI HincII AccI SalI PstI SphI
HindIII
Construction: pUC71K
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ', <-
MCS: EcoRI...HindIII, ->
promoter: lac, <-
replicon: pMB1, <-
marker(s): ampR, <-
Cross references: DNA Seq. Acc.: X02514 |
| Insert |
DNA: genomic DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: D15S63
Gene name: DNA Segment, single copy probes
Contains complete coding sequence?: N
Chromosome: 15; Localization: 15 q11-q12
Type of DNA: genomic
Insert end: HaeIII
Insert end: HaeIII
Insert size (kb): 0.365
Cross references: Insert lengths(kb): 0.3650000095367432 Gene product: DNA Segment, single copy probes [D15S63] Target Gene: DNA Segment, single copy probes |
| Insert Size (kb) |
0.365 |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--2.8, 0.44; PstI--3.2, PvuII--2.5, 0.76; SacI--3.2; XbaI--3.2. Insert detects parent-of-origin specific DNA methylation patterns in the Prader-Willi syndrome/Angelman syndrome chromosomal region (15 q11-q13). An imprinted HpaII and CfoI site in this region results in the maternal and paternal pattern. Patients with Prader-Willi syndrome typically lack the paternal bands. Lack of the maternal bands is indicative of Angelman syndrome. Because of the small probe size, low-stringent post-hybridization washes should be applied. |
| References |
Dittrich B, et al. Characterization of a methylation imprint in the Prader-Willi syndrome chromosome region. Hum. Mol. Genet. 2: 1995-1999, 1993. PubMed: 8111366
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