| 產品名稱 |
BP-TIE2-W2-1 |
| 商品貨號 |
B210934 |
| Designations |
BP-TIE2-W2-1 |
| Species |
Homo sapiens, human |
| Depositors |
R Haronen |
| Vector |
Construct size (kb): 8.899999618530273 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBacPAK8
Intact vector size: 5.538
Type of vector: phagemid
Vector end: XhoI
Vector end: EagI
Cloning sites: BstXI mLuI EcoRV HindIII DraIII ScaI
Polylinker sites: BamHI Sse8387I PstI StuI XhoI BstBI XbaI BglII Asp718I KpnI
Ecl136II SacI EcoRI XmaI SmaI EagI NotI PacI
Features (with orientation and position when available):
promoter: polyhedrin, ->
MCS: multiple cloning site, ->
stop codons: stop codons, ->
marker(s): ampR
replicon: M13
replicon: pMB1
coding sequence: AcMNPV
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: human
Gene symbol: TEK
Gene name: TEK tyrosine kinase, endothelial, mutated
ORF's seq. position: DNA Seq. Acc.: L06139 ()
Contains complete coding sequence?: Y
Chromosome: 9; Localization: 9 p21
Type of DNA: cDNA
Insert 5' end: XhoI
Insert 3' end: EagI
Insert size (kb): 3.4
Cross references: Insert lengths(kb): 3.400000095367432 Gene product: TEK tyrosine kinase, endothelial, mutated [TEK] |
| Insert Size (kb) |
3.400 |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): PstI--8.0, 0.96; BamHI--7.8, 1.1; NcoI/XhoI--5.6, 2.8, 0.5. The insert contains the following restriction sites (approximate kb from the 5' end): PstI--0.9, 1.4; SmaI--2.9. ATCC 87508 contains wild-type receptor tyrosine kinase TIE2 and ATCC 87507 contains a mutant gene that has a point mutation at position 2545 from C to T, resulting in an R849W substitution. |
| References |
Vikkula M, et al. Vascular dysmorphogenesis caused by and activating mutation in the receptor tyrosine kinase TIE2. Cell 87: 1181-1190, 1996. PubMed: 8980225
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