| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--7.6, 5.2, 4.3, 4.0, 3.7, 3.0, 2.4, 2.1, 1.9, 1.6, 1.5, 1.2, 1.1, 0.9, 0.6. The insert reported to contain EcoRI/HindIII fragments of the following sizes (kb): 5.081, 5.054, 4.818, 4.536, 4.225, 4.129, 3.807, 3.424, 2.561, 2.368, 2.111, 2.073, 1.918, 1.646, 1.588, 1.571, 1.526, 1.521, 1.464, 1.165, 1.101, 1.040, 0.971, 0.933, 0.611, 0.586, 0.399, 0.286, 0.243, 0.240, 0.221, 0.141, 0.119, 0.070, 0.058, 0.036, 0.035, 0.023. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. |
