| 產品名稱 |
pBamRM5.0 [NEB699] |
| 商品貨號 |
B211305 |
| Designations |
pBamRM5.0 [NEB699] |
| GenBank Number |
X55285
|
| Species |
Bacillus amyloliquefaciens (Fukumoto) Priest et al. |
| Depositors |
New England Biolabs, Inc., I Schildkraut, New England Biolabs, Inc. |
| Applications |
produces protein modification methylase BamHI produces protein restriction endonuclease BamHI |
| Vector |
Construct size (kb): 4.5 |
| Insert |
DNA: genomic Insert lengths(kb): 1.799999952316284 Gene product: modification methylase BamHI [bamHIC] Target Gene: modification methylase BamHI, restriction endonuclease BamHI |
| Insert Size (kb) |
1.800 |
| Media |
ATCC® Medium 1949: LB medium (ATCC medium 1065) with 50 mcg/ml chloramphenicol and 100 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of pBamRM5.0 give the following sizes (kb): HindIII/SmaI--5.0, 4.4; HindIII--5.0, 4.4; PvuII--4.5, 4.5, 0.4; EcoRI--9.5. Restriction digests of pBamM1.8 give the following sizes (kb)(faint): HindIII/SmaI--4.4; HindIII--4.4; PvuII--2.4, 2.1; EcoRI--4.5. |
| References |
Brooks JE. Method for producing the BamHI restriction endonuclease and methylase. US Patent 5,137,823 dated Aug 11 1992
Brooks JE, et al. Cloning the BamHI restriction modification system. Nucleic Acids Res. 17: 979-997, 1989. PubMed: 2537955
Brooks JE, et al. Characterization of the cloned BamHI restriction modification system: its nucleotide sequence, properties of the methylase, and expression in heterologous hosts. Nucleic Acids Res. 19: 841-850, 1991. PubMed: 1901989
|
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
