| Vector |
Construct size (kb): 4.401999950408936 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ', <-
primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, <-
promoter: lac, <-
primer site: M13 reverse, <-
replicon: pMB1
marker(s): ampR, <-
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: D18S950E
Gene name: DNA Segment, single copy, expressed
Contains complete coding sequence?: U
Chromosome: 18
Tissue: BL29 cell line
Disease: Burkitt's lymphoma
Type of DNA: cDNA
Insert end: Modification: EcoRI linker
Insert end: Modification: EcoRI linker
Insert size (kb): 1.502
Cross references: DNA Seq. Acc.: T46905; EST: EST014; Primer: T7; THC id:
THC112095
Nucleotides 1-1502 of the insert correspond to
nucleotides 1-1502 of T46905. Insert lengths(kb): 1.501999974250794 Tissue: BL29 cell line Burkitt's lymphoma Disease: Burkitt's lymphoma Gene product: DNA Segment, single copy, expressed [D18S950E] |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 0.9, 0.6; BamHI--4.3; HindIII--4.3; BglI--3.2, 1.2; PvuII--2.3, 1.3, 0.5, 0.2. The insert contains the following restriction sites (approximate kb from the T7 end of the insert): NcoI--0.71; EcoRI--0.91, PvuII--1.2, 1.3; PstI--1.3. |
