| Vector |
Construct size (kb): 6.800000190734863 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pUC18
Intact vector size: 2.686
Type of vector: plasmid
Vector end: BamHI
Vector end: BamHI
Cloning sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI SmaI KpnI SacI
EcoRI
Polylinker sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI SmaI KpnI SacI
EcoRI
Construction: pUC71K
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ', <-
MCS: HindIII...EcoRI, ->
promoter: lac, <-
replicon: pMB1, <-
marker(s): ampR, <-
Cross references: DNA Seq. Acc.: L08752 |
| Insert |
DNA: genomic DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: TP53
Genomic copy number: unique
Gene name: tumor protein p53 (Li-Fraumeni syndrome)
Contains complete coding sequence?: U
Chromosome: 17; Localization: 17 p13.1
Type of DNA: genomic
Insert end: BamHI
Insert end: BamHI
Insert size (kb): 4.1
Cross references: Insert lengths(kb): 4.099999904632568 Gene product: tumor protein p53 (Li-Fraumeni syndrome)( tumor protein p53 (Li-Fraumeni syndrome), p53 cellular tumor antigen) [TP53] Alleles: D2, C2, C1, A1, A1, B2, B1, D1, A2, A2 |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--4.1, 2.7; BglI--3.9, 1.1, 0.9, 0.8; EcoRI--6.8; AvaI--3.9, 2.3, 0.5; KpnI--6.8. Not recommended for detection of p53 transcripts. The probe was pre-associated with total human DNA. Otherwise, RFLPs were observed under normal hybridization and wash stringencies. Subcloned from a cosmid isolated by hybridization with a cDNA for p53. Enzymes(s) not detecting polymorphism: BglII, EcoRI, HindIII, MspI, PstI, PvuII, RsaI, TaqI. |
