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pRML2
pRML2
規格:
貨期:
編號:B212232
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱 pRML2
商品貨號 B212232
Designations pRML2
Depositors F Spencer
Biosafety Level 1
Vector Information
Size (kb): 5.0999999046325680
Vector: pRML2 (plasmid)
Promoters: Promoter for in vitro transcription T7
Construction: pBluescript + URA3 + ARSH4
Marker(s): ampR

Features:
marker(s): URA3
marker(s): ampR
promoter for in vitro transcription: T7
replicon: ARSH4
replicon: ori
restriction site: SalI
coding sequence: TEL
Applications
cloning vector
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--5.1; EcoRI--5.1; NotI--5.1; XbaI--5.1; EcoRI/XbaI--4.5, 0.5.
YAC cloning vector. The plasmid was from pBluescript, and includes a URA3 selectable marker, yeast origin of replication ARSH4, a distal polylinker to support plasmid rescue of insert ends, T7 promoter, and a cloning polylinker for library production.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37°C
References

Spencer F, et al. Targeted recombination-based cloning and manipulation of large DNA segments in yeast. Methods 5: 161-175, 1993.

Smith DR, et al. Copy number amplification of yeast artifical chromosomes. Methods Enzymol. 216: 603-614, 1992. PubMed: 1336106

Zhong TP, et al. Zebrafish genomic library in yeast artifical chromosomes. Genomics 48: 136-138, 1998. PubMed: AMBIGUOUS 95

Haldi ML, et al. A comprehensive large-insert yeast artificial chromosome library for physical mapping of the mouse genome. Mamm. Genome 7: 767-769, 1996. PubMed: 8854865

Shipped freeze-dried
Shipping Information in E. coli containing the plasmid
梅經理 17280875617 1438578920
胡經理 13345964880 2438244627
周經理 17757487661 1296385441
于經理 18067160830 2088210172
沈經理 19548299266 2662369050
李經理 13626845108 972239479
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