| 產品名稱 |
Caviid herpesvirus 2 |
| 商品貨號 |
B212338 |
| Classification |
Herpesviridae, Unassigned |
| Deposited As |
Deposited as Guinea pig salivary gland virus |
| Agent |
Caviid herpesvirus 2 |
| Strain |
22122 |
| Biosafety Level |
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation |
Submaxillary glands from a guinea pig |
| Product Format |
frozen 1 mL per vial |
| Storage Conditions |
-70°C or colder |
| Effect on Host |
CPE, refractile rounding and sloughing |
| Recommended Host |
Guinea pig lung (ATCC® CCL-158™) |
| Growth Conditions |
Temperature: 37°C
Recommendations for Infection: For best results, infection should be performed on a 80-90% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2). Wash monolayer with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 9-21 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% or greater of the monolayer. |
| Name of Depositor |
MJ Collins, Jr., Microbiological Associates, Inc. |
| Source |
Submaxillary glands from a guinea pig |
| References |
Hartley JW, et al. Serial propagation of the guinea pig salivary gland virus in tissue culture. Proc. Soc. Exp. Biol. Med. 96: 281-285, 1957. PubMed: 13485080
|
| Cross References |
Nucleotide (GenBank) :
AB592928.1
Caviid herpesvirus 2 DNA, complete genome, strain: 22122
|
