| 產(chǎn)品名稱 | Chlamydia trachomatis Serovar Ba |
|---|---|
| 商品貨號 | B212517 |
| Classification | Chlamydiaceae, Chlamydia |
| Deposited As | Trachoma serotype ba |
| Agent | Chlamydia trachomatis Serovar Ba |
| Strain | Apache-2 |
| Applications | Sexually Transmitted Disease Research |
| Biosafety Level | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format | frozen 1 mL per vial |
| Storage Conditions | -70°C or colder |
| Images |  |
| Comments | FBS used to culture Chlamydia must be prescreened to verify that the serum does not contain antibodies to Chlamydia or other factors that would interfere with growth.
Next-generation sequencing (NGS) at ATCC on the McCoy cell line (ATCC® CRL-1696™) used as the host has shown the presence of Mus Musculus mobilized endogenous polytropic provirus and Murine leukemia virus. |
| Effect on Host | Intracellular inclusion bodies visualized by fluorescent staining |
| Recommended Host | McCoy cells (ATCC CRL-1696?) |
| Growth Conditions | Temperature: 36°C
Recommendations for Infection: For best results, infection should be performed on a 80-100% confluent, 24-48 hour old cellular monolayer. Sonicate seed material for 20 seconds at approximately 240W to disrupt cells. Prepare dilution of chlamydia in minimum amount of volume (e.g. 1 mL per 25 cm2). Remove cell growth medium and inoculate with disrupted material. For adsorption, centrifuge at 1500 x g at 25°C for 1 hour. End adsorption by adding agent growth medium.
Incubation: Incubate infected culture for 72 hours at 36°C in a humidified 5% CO2 atmosphere.
Preservation: Controlled rate freeze. |
| Name of Depositor | E Jawetz |
| Special Collection | ATCC |
| Source | Eye of an Apache Indian child from San Carlos, Arizona |
| References | Hanna L, et al. Trachoma viruses isolated in United States. I. Growth in embryonated eggs. Proc. Soc. Exp. Biol. Med. 104: 142-145, 1960. PubMed: 14399529 |
| 梅經(jīng)理 | 17280875617 | 1438578920 |
| 胡經(jīng)理 | 13345964880 | 2438244627 |
| 周經(jīng)理 | 17757487661 | 1296385441 |
| 于經(jīng)理 | 18067160830 | 2088210172 |
| 沈經(jīng)理 | 19548299266 | 2662369050 |
| 李經(jīng)理 | 13626845108 | 972239479 |
